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By Alton Meister

Advances in Enzymology and comparable components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative studies of the most recent discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unequalled view of the historic improvement of enzymology. The sequence deals researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic approach, and their software in either the laboratory and undefined. every one quantity within the sequence positive aspects contributions by way of best pioneers and investigators within the box from worldwide. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.

With its wide variety of issues and lengthy ancient pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just through scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition via any scientist attracted to the invention of an enzyme, its homes, and its functions.

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Extra resources for Advances in Enzymology and Related Areas of Molecular Biology, Volume 13

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25 25 A. Useful Equations.. . . . . . . . . . . . . . . . . . . . B. Determination of F Directions, Using Two Tracers. 27 . . . . . . . . . , . _ . 29 . IV. The Components of the A. Meaning of Free Exchange Diffusion, and Active 29 Transport . . . . . . . . . . . . . . . . . . . . . . B. Equations Useful for Distinguishing between Free Diffusion and 31 Diffusion in Chemical Combination. V. Relation between the Flux Ratio of a Substance and Its Electrochemical Activities in Inside and Outside Solutions.

48 56 B. Gastric Mucosa . . . . . . . . . . . . . . . . . . . . . 58 C. Muscle ... . . . . . . . . . . . . . . . . D. Nerve.. 60 VIII. Concluding Remarks. . . . . . . . . . . . . . . . . . . . . 63 References. . . . . . . . . , . , . . . . . . . . . . . . . . . 64 I. Introduction To the enzymologist permeability problems are of interest not only on account of the trivial fact that permeation processes often influence apparent reaction rates in cells, tissue slices, and surviving organs; the importance of enzymic processes for the uptake of phosphate in cells has been realized for some time, and it is becoming increasingly clear that “secretion” or active transport, even of inorganic ions like Na, C1, K, and so on, can only be explained as the result of chemical binding during the passage through the living membrane to carrier molecules (5,64).

It was also the first living organ the permeability of which was studied by means of heavy water (Hevesy, Hofer, and Krogh, 1935 (18)). The permeability to heavy water was determined both on the isolated skin and on the whole animal. The net uptake of water in animals immersed in distilled water was measured as the sum of the urine produced and the gain in weight. The authors assumed that the flux ratio of water ought to be equal to the ratio between the water concentrations in the inside and outside media.

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